不同肤色山羊皮肤组织形态学及黑色素生成相关基因的表达分析

doi:10.11843/j.issn.0366-6964.2015.09.006

Abstract

Abstract:

The objective of this study was to elucidate the characteristics in histomorphology and the potential molecular mechanisms underlying the dermal hyperpigmentation in Youzhou Dark-skin goat.First，we analyzed the characteristics of histomorphology in 100 d-fetal skin in Youzhou Dark-skin goat(dark skin) and Yudong White goat(normal skin).Then we examined the mRNA expression of Pax3，Mitf and Tyr in skin and B16 cells in the 2 breeds.The results showed that：1) There were significant differences in the skin pigmentation between breeds at fetus skin rapid development stage；2) The skin mRNA levels of Pax3 and Tyr were significantly higher in Youzhou Dark-skin goat than that in Yudong White goat(P<0.001)，whereas no significant difference was observed in Mitf expression between the 2 breeds(P>0.05)；3) The expression of Pax3 was decreased(P<0.001)，Tyr was increased(P<0.01) during the B16 cell differentiation，as compared with the proliferating B16 cells.The results indicate that：1) There might be diversity in migration pathway of the dermal melanocytes between the dark-skin and normal skin goats during the early stage of skin development differentiation；2) Downregulation of Pax3 and upregulation of Tyr are indispensible for melanogenesis in melanocytes；3) The high expression of Pax3 and Tyr are associated with the dermal hyperpigmentation in Youzhou Dark-skin goat.

CLC Number:

S827
S813.3

REN Hang-xing，WANG Gao-fu，LU Jian，LI Jie，JIANG Jing，LIU Liang-jia，ZHOU Peng. The Characteristic Histomorphology of Various Skin Colors and Expression of Genes Involved in Melanogenesis in Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1525-1531.

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The Characteristic Histomorphology of Various Skin Colors and Expression of Genes Involved in Melanogenesis in Goats

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